Description
| Reference | ESCIT138 |
|---|---|
| Size | 20ug |
| Molecular Weight | 27.5 KDa |
| Purity | >95% by SDS-PAGE. |
| Endotoxin | <1 EU/µg |
| Biological Activity | Loaded Anti-Human FOLR1 mAb-Fc(Cat#NC072) on Protein A Biosensor, can bind Human FOLR1-His-Avi(Cat#CY16) with an affinity constant of 8.38 nM as determined in BLI assay. |
Other names: Folate recESCItor alpha;FR-alpha;Adult folate-binding protein;FBP;Folate recESCItor 1;Folate recESCItor;Ovarian tumor-associated antigen MOv18;FOLR1
Redissolve: Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Â
Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Shipping Condition: Ambient temperature.
Background: Folate recESCItor alpha(FOLR) belongs to the folate recESCItor family, and is primarily expressed in tissues of ESCIithelial origin. It is also expressed in kidney, lung and cerebellum. The secreted form is derived from the membrane-bound form either by cleavage of the GPI anchor, or/and by proteolysis catalyzed by a metalloprotease. FOLR1 binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. It has high affinity for folate and folic acid analogs at neutral pH. Exposure to slightly acidic pH after recESCItor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release. It is required for normal embryonic development and normal cell proliferation.