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Recombinant Human APRIL (N-Flag-His)

Recombinant Human A proliferation-inducing Ligand is produced by our Mammalian expression system and the target gene encoding Lys112-Leu250 is expressed with a His, Flag tag at the N-terminus.

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Description

Reference ESCIT169
Size 10ug
Molecular Weight 50 KDa
Purity >95% by SDS-PAGE.
Endotoxin <1 EU/µg
Biological Activity Immobilized Human APRIL -Flag-His(Cat#CU89) at 1μg/ml (100 μl/well) can bind Cynomolgus BCMA-Fc(Cat#CP59).The ED50 of Cynomolgus BCMA-Fc(Cat#CP59) is 4.23 ng/ml.

Other names: Tumor necrosis factor ligand superfamily member 13; A proliferation-inducing ligand; APRIL; TNF- and APOL-related leukocyte expressed ligand 2; TALL-2; TNF-related death ligand 1; TRDL-1; CD256; TNFSF13

 

Redissolve: Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 

 

Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

 

Shipping Condition: Ambient temperature.

 

Background: APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily. It is synthesized as a 32 kDa proprotein which is cleaved by furin in the Golgi to release the active 17 kDa soluble molecule. Secreted human APRIL, which consists almost entirely of a single TNF homology domain, shares 85% amino acid sequence identity with mouse and rat APRIL. Both APRIL and its close relative BAFF bind and signal through the TNF superfamily recESCItors TACI and BCMA, while BAFF additionally functions through BAFF R. APRIL binds to hESCIaran sulfate proteoglycans (HSPGs) indESCIendently of its binding to TACI and BCMA. APRIL can form bioactive heterotrimers with BAFF, and these circulate in the serum of patients with rheumatic immune disorders. APRIL enhances the proliferation and survival of plasma cells and also promotes T cell-dESCIendent humoral responses. APRIL levels are elevated in the serum during coronary artery disease, and it is also elevated in many cancers primarily due to expression by tumor-infiltratin neutrophils.