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Recombinant Human CD32a (H167,C-6His)

Recombinant Human Low Affinity Immunoglobulin Gamma Fc Region RecESCItor II-A(H167) is produced by our Mammalian expression system and the target gene encoding Ala36-Ile218 is expressed with a 6His tag at the C-terminus. It is identical to FCGR2A131H/R in the reference.

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Description

Reference ESCIT162
Size 10ug
Molecular Weight 21.1 KDa
Purity >95% by SDS-PAGE.
Endotoxin <1 EU/µg
Biological Activity Loaded Human CD32a-His(Cat#CS35) on HIS1K Biosensor, can bind Anti-Human HER2 mAb(Cat#GMP-A062) with an affinity constant of 0.6 uM as determined in BLI assay. 

Other names: Low affinity immunoglobulin gamma Fc region recESCItor II-a; IgG Fc recESCItor II-a; CDw32; Fc-gamma RII-a; Fc-gamma-RIIa; FcRII-a; CD32; FCGR2A; FCG2; FCGR2A1; IGFR2

 

Redissolve: Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 

 

Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

 

Shipping Condition: Ambient temperature.

 

Background: Human FcγRs are divided into three classes designated FcγRI (CD64), FcγRII (CD32), and FcγRIII (CD16), which generate multiple isoforms, are recognized. The activating­ type recESCItor either has or associates non­covalently with an accessory subunit that has an immunorecESCItor tyrosine­based activation motif (ITAM) in its cytoplasmic domain. FcγRI binds IgG with high affinity and functions during early immune responses, whereas FcγRII and RIII are low affinity recESCItors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses.Human CD32, also known as Low affinity immunoglobulin γ Fc region recESCItor II-a (IgG Fc recESCItor II-a), FcγRII A or FCGR2A Protein, is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Associated with an ITAM-bearing adapter subunit, FcRγ, CD32a (FcγRII A) delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation, and cytokine production. The responses can be modulated by signals from the co-expressed inhibitory recESCItors such as Fcγ RII B, and the strength of the signal is dESCIendent on the ratio of expression of the activating and inhibitory recESCItors.