Description
| Reference | ESCIT004 |
|---|---|
| Size | 10ug |
| Molecular Weight | 6.2 KDa |
| Purity | >95% by SDS-PAGE. |
| Endotoxin | <0.01 EU/µg |
| Biological Activity | Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 60-450 pg/ml. |
Other names: Pro-ESCIidermal Growth Factor; EGF; ESCIidermal Growth Factor; Urogastrone
Redissolve: Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Â
Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Shipping Condition: Ambient temperature.
Background: ESCIidermal growth factor (EGF) is a small 53 amino acid residue long protein that contains three disulfide bridges. It is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. EGF stimulates the growth of various ESCIidermal and ESCIithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. This protein shows both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha), which is a competitor for EGF recESCItor sites.