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Recombinant Human NEDD8 (N-6His-SUMO)

Recombinant Human Neural Precursor Cell Expressed Developmentally Down-regulated Protein 8 is produced by our E.coli expression system and the target gene encoding Met1-Gly76 is expressed with a 6His, SUMO tag at the N-terminus.

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Description

Reference ESCIT278
Size 10ug
Molecular Weight 20.9 KDa
Purity >95% by SDS-PAGE.
Endotoxin <1 EU/µg
Biological Activity

Other names: Neural precursor cell expressed developmentally down-regulated protein 8; NEDD8; Neddylin; Ubiquitin-like protein Nedd8

 

Redissolve: Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 

 

Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

 

Shipping Condition: Ambient temperature.

 

Background: Human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The only known substrates of NEDD8 modification are the cullin subunits of SCF ubiquitin E3 ligases. The NEDDylation of cullins is critical for the recruitment of E2 to the ligase complex, thus facilitating ubiquitin conjugation. NEDD8 modification has therefore been implicated in cell cycle progression and cytoskeletal regulation.