Recombinant Mouse TfR (N-8His)

Description

Other names: Transferrin recESCItor protein 1; TR; TfR; TfR1; Trfr; CD71; Tfrc

Redissolve: Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 

Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Shipping Condition: Ambient temperature.

Background: Transferrin recESCItor protein 1 (TFRC) belongs to the pESCItidase M28 family that is synthesized as a 172 amino acid (aa). TFRC regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3′-UTR. It binds one transferrin or HFE molecule per subunit and binds the HLA class II histocompatibility antigen, DR1. It Interacts with SH3BP3 and STEAP3, facilitates TFRC endocytosis in erythroid precursor cells. Cellular uptake of iron occurs via recESCItor-mediated endocytosis of ligand-occupied transferrin recESCItor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-recESCItor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its recESCItor. Transferrin recESCItor is necessary for development of erythrocytes and the nervous system. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. It positively regulates T and B cell proliferation through iron uptake.