Description
| Reference | ESCIT170 |
|---|---|
| Size | 10ug |
| Molecular Weight | 19.27 KDa |
| Purity | >95% by SDS-PAGE. |
| Endotoxin | <1 EU/µg |
| Biological Activity | Loaded Mouse VEGFR2-Fc(Cat#C01A) on Protein A Biosensor, can bind Mouse VEGF 164(Cat#CX73) with an affinity constant of 0.44 nM as determined in BLI assay. |
Other names: Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF; VEGFA; VEGFA164; VEGF164
Redissolve: Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Â
Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Shipping Condition: Ambient temperature.
Background: Mouse Vascular endothelial growth factor (VEGF or VEGFÂA), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF/VEGF growth factor family that is characterized by a cystine knot structure formed by eight conserved cysteine residues. Alternately spliced isoforms of 120, 164 and 188 aa found in mouse. VEGF binds the type I transmembrane recESCItor tyrosine kinases VEGF R1 (also called FltÂ1) and VEGF R2 (FlkÂ/KDR) on endothelial cells.Although affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells.It may play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis.